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KMID : 1007520030120020133
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Food Science and Biotechnology
2003 Volume.12 No. 2 p.133 ~ p.136
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Sequencing, Cloning and Expression of CP4EPSPS Roundup Ready Soybean Insert
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Son, Dae-Yeul
Ahn, Kang-Mo/Lee, Sang Il
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Abstract
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Results of the CP4EPSPS gene sequence in the transgenic Roundup Ready soybean, and cloning and expression of CP4EPSPS protein using bacterial expression system are described. Our sequencing results show that DNA rearrangement occurred between 3´NOS terminator and the genomic plant DNA/ Expressed CP4EPSPS protein was isolated using simple two steps of DEAE column, and can be used as a source material for the allergic risk assessment of newly introduced foreign protein in soybean and for the production of mono- and/or poly-clonal antibodies.
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